Validation Data Gallery

Filter: AllWB

WB analysis of mouse pancreas using 19889-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19889-1-AP (COX4NB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 19889-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 19889-1-AP (COX4NB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 19889-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19889-1-AP (COX4NB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of human placenta using 19889-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 19889-1-AP (COX4NB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 19889-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 19889-1-AP (COX4NB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

COX4NB Polyclonal antibody

COX4NB Polyclonal Antibody for WB, ELISA

Cat No. 19889-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

C16orf2, C16orf4, COX4 neighbor, COX4AL, COX4NB, EMC8, FAM158B, Neighbor of COX4, NOC4, Protein FAM158B

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

mouse pancreas tissue, COLO 320 cells, human placenta tissue, mouse liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IP	See 1 publications below

Product Information

19889-1-AP targets COX4NB in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	COX4NB fusion protein Ag13720 相同性解析による交差性が予測される生物種
Full Name	COX4 neighbor
Calculated molecular weight	210 aa, 24 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC001472
Gene Symbol	COX4NB
Gene ID (NCBI)	10328
RRID	AB_2878618
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O43402
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for COX4NB antibody 19889-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	IP	Mol Cell WNK1 is an assembly factor for the human ER membrane protein complex. Authors - Tino Pleiner View Article
human	WB	PLoS Pathog A specific EMC subunit supports Dengue virus infection by promoting virus membrane fusion essential for cytosolic genome delivery. Authors - Parikshit Bagchi View Article
mouse	WB	Nat Methods Global analysis of endogenous protein disorder in cells Authors - Shouxiang Zhang View Article

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企業情報

抗体

免疫アッセイ

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サポートツール・試薬

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse pancreas using 19889-1-AP

WB analysis of human placenta using 19889-1-AP

WB analysis of mouse liver using 19889-1-AP