Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
11441-1-AP targets COX15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2004 Product name: Recombinant human COX15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-410 aa of BC002382 Sequence: MQRLLFPPLRALKGRQYLPLLAPRAAPRAQCDCIRRPLRPGQYSTISEVALQSGRGTVSLPSKAAERVVGRWLLVCSGTVAGAVILGGVTRLTESGLSMVDWHLIKEMKPPTSQEEWEAEFQRYQQFPEFKILNHDMTLTEFKFIWYMEYSHRMWGRLVGLVYILPAAYFWRKGWLSRGMKGRVLALCGLVCFQGLLGWYMVKSGLEEKSDSHDIPRVSQYRLAAHLGSALVLYCASLWTSLSLLLPPHKLPETHQLLQLRRFAHGTAGLVFLTALSGAFVAGLDAGLVYNSFPKMGESWIPEDLFTFSPILRNVFENPTMVQFDHRILGITSVTAITVLYFLSRRIPLPRRTKMAAVTLLALAYTQVGLGISTLLMYVPTPLAATHQSGSLALLTGALWLMNELRRVPK 相同性解析による交差性が予測される生物種 |
| Full Name | COX15 homolog, cytochrome c oxidase assembly protein (yeast) |
| Calculated molecular weight | 410 aa, 46 kDa |
| Observed molecular weight | 44-55 kDa |
| GenBank accession number | BC002382 |
| Gene Symbol | COX15 |
| Gene ID (NCBI) | 1355 |
| RRID | AB_10792251 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7KZN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COX15 belongs to the COX15/CtaA family. It may be involved in the biosynthesis of heme A. COX15 has 2 isoforms COX15.1 and COX15.2. The MW in SDS-PAGE is between 44kd and 55kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for COX15 antibody 11441-1-AP | Download protocol |
| WB protocol for COX15 antibody 11441-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages. | ||
Int J Mol Sci Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling | ||
Biochim Biophys Acta Mol Basis Dis Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure. | ||
J Nanobiotechnology Modulation of alveolar macrophage and mitochondrial fitness by medicinal plant-derived nanovesicles to mitigate acute lung injury and viral pneumonia |
