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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CORO7 Polyclonal antibody (31425-1-AP)

    WB analysis using 31425-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31425-1-AP (CORO7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CORO7 Polyclonal antibody (31425-1-AP)

    IP experiment of HeLa using 31425-1-AP

    IP result of anti-CORO7 (IP:31425-1-AP, 4ug; Detection:31425-1-AP 1:2000) with HeLa cells lysate 980 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CORO7 Polyclonal antibody (31425-1-AP)

    IHC staining of human ovary cancer using 31425-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31425-1-AP (CORO7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CORO7 Polyclonal antibody

CORO7 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 31425-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

CRN7, Coronin-7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, MDA-MB-231 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31425-1-AP targets CORO7 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CORO7 fusion protein Ag35016 相同性解析による交差性が予測される生物種
Full Name coronin 7
Calculated molecular weight101 kDa
Observed molecular weight 100 kDa
GenBank accession numberNM_024535
Gene Symbol CORO7
Gene ID (NCBI) 79585
RRIDAB_3669976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP57737
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CORO7 also known as CRN7 belongs to the WD repeat coronin family, and functions as an F-actin regulator directing anterograde Golgi to endosome transport(PMID: 15327992).

Protocols

Product Specific Protocols
WB protocol for CORO7 antibody 31425-1-APDownload protocol
IHC protocol for CORO7 antibody 31425-1-APDownload protocol
IP protocol for CORO7 antibody 31425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols