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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CORO1B Polyclonal antibody (31091-1-AP)

    WB analysis using 31091-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31091-1-AP (CORO1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CORO1B Polyclonal antibody (31091-1-AP)

    IHC staining of mouse liver using 31091-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31091-1-AP (CORO1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CORO1B Polyclonal antibody (31091-1-AP)

    IHC staining of human ovary cancer using 31091-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31091-1-AP (CORO1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CORO1B Polyclonal antibody

CORO1B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31091-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Coronin-2, Coronin-1B, CORONIN 2, Coronin 1B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells, MDA-MB-453 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse liver tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31091-1-AP targets CORO1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CORO1B fusion protein Ag34813 相同性解析による交差性が予測される生物種
Full Name coronin, actin binding protein, 1B
Calculated molecular weight 498 aa, 54 kDa
Observed molecular weight68 kDa
GenBank accession numberBC006449
Gene Symbol CORO1B
Gene ID (NCBI) 57175
RRIDAB_3669849
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BR76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CORO1B (Coronin 1B) is an actin-binding protein that controls actin networks at classical lamellipodia (PMID: 32850828). Coro1B localizes to the plasma membrane, regulates lamellipodia formation, and when phosphorylated, it can no longer bind ARP2/3 and inhibit its actin nucleation abilities (PMID: 22619279).

Protocols

Product Specific Protocols
WB protocol for CORO1B antibody 31091-1-APDownload protocol
IHC protocol for CORO1B antibody 31091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols