Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | Jurkat cells, mouse brain tissue, mouse thymus tissue, rat thymus tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00049 targets CORO1A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | coronin, actin binding protein, 1A |
| Calculated molecular weight | 461 aa, 51 kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | BC110374 |
| Gene Symbol | CORO1A |
| Gene ID (NCBI) | 11151 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | P31146 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}} |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CORO1A antibody RMX00049 | Download protocol |
| IHC protocol for CORO1A antibody RMX00049 | Download protocol |
| IP protocol for CORO1A antibody RMX00049 | Download protocol |
| WB protocol for CORO1A antibody RMX00049 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |