Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue, mouse liver tissue, rat liver tissue, mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85890-1-RR targets COQ5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag10203 Product name: Recombinant human COQ5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-327 aa of BC107874 Sequence: MAAPGSCALWSYCGRGWSRAMRGCQLLGLRSSWPGDLLSARLLSQEKRAAETHFGFETVSEEEKGGKVYQVFESVAKKYDVMNDMMSLGIHRVWKDLLLWKMHPLPGTQLLDVAGGTGDIAFRFLNYVQSQHQRKQKRQLRAQQNLSWEEIAKEYQNEEDSLGGSRVVVCDINKEMLKVGKQKALAQGYRAGLAWVLGDAEELPFDDDKFDIYTIAFGIRNVTHIDQALQEAHRVLKPGGRFLCLEFSQVNNPLISRLYDLYSFQVIPVLGEVIAGDWKSYQYLVESIRRFPSQEEFKDMIEDAGFHKVTYESLTSGIVAIHSGFKL 相同性解析による交差性が予測される生物種 |
| Full Name | coenzyme Q5 homolog, methyltransferase (S. cerevisiae) |
| Calculated molecular weight | 327 aa, 37 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC107874 |
| Gene Symbol | COQ5 |
| Gene ID (NCBI) | 84274 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5HYK3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COQ5 catalyzes the only C-methylation involved in the biosynthesis of coenzyme Q (Q or ubiquinone) in humans and yeast Saccharomyces cerevisiae. In humans, mutations in several COQ genes cause primary Q deficiency, and a decrease in Q biosynthesis is associated with mitochondrial, cardiovascular, kidney and neurodegenerative diseases (PMID: 25152161). Endogenous human COQ5 protein primarily existed as a mature form (m-COQ5, ~32 kDa) without the mitochondrial targeting sequence (MTS) in the mitochondria of human cells, in addition to the minor precursor form (p-COQ5, 37 kDa) of the full-length protein (PMID: 27155576).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COQ5 antibody 85890-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |