Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, Transfected HEK-293 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
16654-1-AP targets COQ4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10089 Product name: Recombinant human COQ4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-265 aa of BC011895 Sequence: MATLLRPVLRRLCGLPGLQRPAAEMPLRARSDGAGPLYSHHLPTSPLQKALLAAGSAAMALYNPYRHDMVAVLGETTGHRTLKVLRDQMRRDPEGAQILQERPRISTSTLDLGKLQSLPEGSLGREYLRFLDVNRVSPDTRAPTRFVDDEELAYVIQRYREVHDMLHTLLGMPTNILGEIVVKWFEAVQTGLPMCILGAFFGPIRLGAQSLQVLVSELIPWAVQNGRRAPCVLNLYYERRWEQSLRALREELGITAPPMHVQGLA 相同性解析による交差性が予測される生物種 |
| Full Name | coenzyme Q4 homolog (S. cerevisiae) |
| Calculated molecular weight | 265 aa, 30 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC011895 |
| Gene Symbol | COQ4 |
| Gene ID (NCBI) | 51117 |
| RRID | AB_2878296 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3A0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human coenzyme Q4 (COQ4), as a component of the coenzyme Q biosynthetic pathway, is required for the oxidative decarboxylation of the C1 carbon of Coenzyme Q in eukaryotic cells (PMID: 38295803). COQ4 deficiency manifests as an early-onset neurodegenerative disorder. COQ4 encodes a ubiquitously expressed 265-amino-acid protein that is peripherally associated with the mitochondrial inner membrane on the matrix side (PMID: 25658047, 34656997)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for COQ4 antibody 16654-1-AP | Download protocol |
| IP protocol for COQ4 antibody 16654-1-AP | Download protocol |
| WB protocol for COQ4 antibody 16654-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1 | ||
EMBO Mol Med β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice. | ||
Redox Biol The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency. | ||
Ann Clin Transl Neurol 4-Hydroxybenzoic acid restores CoQ10 biosynthesis in human COQ2 deficiency. | ||
J Med Genet Human COQ4 deficiency: delineating the clinical, metabolic and neuroimaging phenotypes. | ||
Biochim Biophys Acta Bioenerg Characterization of human mitochondrial PDSS and COQ proteins and their roles in maintaining coenzyme Q10 levels and each other's stability. |
