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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COQ10A Polyclonal antibody (29496-1-AP)

    WB analysis using 29496-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29496-1-AP (COQ10A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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COQ10A Polyclonal antibody

COQ10A Polyclonal Antibody for WB, ELISA
Cat No. 29496-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Coenzyme Q-binding protein COQ10 homolog A, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29496-1-AP targets COQ10A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COQ10A fusion protein Ag31534 相同性解析による交差性が予測される生物種
Full Name coenzyme Q10 homolog A (S. cerevisiae)
Calculated molecular weight28KD
Observed molecular weight20 kDa
GenBank accession numberNM_144576
Gene Symbol COQ10A
Gene ID (NCBI) 93058
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MF6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COQ10A is a human gene that encodes a protein involved in the biosynthesis of coenzyme Q10 (CoQ10), also known as ubiquinone. CoQ10 is a lipid-soluble antioxidant and a critical component of the mitochondrial electron transport chain, where it plays a key role in cellular energy production (ATP synthesis). Mutations or deficiencies in COQ10A have been implicated in primary CoQ10 deficiency syndromes, which can manifest as mitochondrial disorders such as encephalomyopathy, nephropathy, and Leigh syndrome.

Protocols

Product Specific Protocols
WB protocol for COQ10A antibody 29496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols