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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPZ1 Recombinant monoclonal antibody (86014-1-RR)

    WB analysis using 86014-1-RR (same clone as 86014-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86014-1-RR (COPZ1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86014-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COPZ1 Recombinant monoclonal antibody (86014-1-RR)

    IF Staining of HeLa using 86014-1-RR (same clone as 86014-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using COPZ1 antibody (86014-1-RR, Clone: 250388F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86014-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86014-1-RR

    Affinity and Kinetic Characterization of 86014-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86014-1-RR against Human COPZ1 were performed. The affinity constant is 1.45 nM.

COPZ1 Recombinant monoclonal antibody, PBS Only

COPZ1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86014-1-PBS
Clone No.250388F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CGI 120, CGI-120, Coatomer subunit zeta 1, Coatomer subunit zeta-1, COPZ

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86014-1-PBS targets COPZ1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag14234

Product name: Recombinant human COPZ1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 31-84 aa of BC002849

Sequence: YDDTYPSVKEQKAFEKNIFNKTHRTDSEIALLEGLTVVYKSSIDLYFYVIGSSY

相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit zeta 1
Calculated molecular weight 177 aa, 20 kDa
Observed molecular weight15-20 kDa
GenBank accession numberBC002849
Gene Symbol COPZ1
Gene ID (NCBI) 22818
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP61923
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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