Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, human testis tissue, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32695-1-AP targets COPS7B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38543 Product name: Recombinant human COPS7B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 100-264 aa of NM_022730.3 Sequence: TIVSLASRMKCIPYSVLLKDLEMRNLRELEDLIIEAVYTDIIQGKLDQRNQLLEVDFCIGRDIRKKDINNIVKTLHEWCDGCEAVLLGIEQQVLRANQYKENHNRTQQQVEAEVTNIKKTLKATASSSAQEMEQQLAERECPPHAEQRQPTKKMSKVKGLVSSRH* 相同性解析による交差性が予測される生物種 |
| Full Name | COP9 constitutive photomorphogenic homolog subunit 7B (Arabidopsis) |
| Calculated molecular weight | 30 kDa，264 aa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | NM_022730.3 |
| Gene Symbol | COPS7B |
| Gene ID (NCBI) | 64708 |
| RRID | AB_3742712 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H9Q2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COP9 signalosome complex subunit 7b (COPS7B, also known as CSN7B) is a component of the ribo-interactome that interacts with ribosomes to facilitate ribosome biogenesis and mRNA translation initiation (PMID: 27903243). Increased expression of COPS7B mediated by IGF2BP3 elevates the translational efficiency of genes enriched in mRNA translation and ribosome biogenesis pathways, promoting protein synthesis and driving progression in colorectal cancer (PMID: 37560971).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COPS7B antibody 32695-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |