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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COPG2 Polyclonal antibody

COPG2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16111-1-AP

Synonyms

2 COP, Coatomer subunit gamma 2, COPG2, Gamma 2 coat protein, Gamma 2 COP

Validation Data Gallery

Applications:
  • WB analysis of mouse heart using 16111-1-AP

    WB analysis of mouse heart using 16111-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16111-1-AP (COPG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 16111-1-AP

    WB analysis of human heart using 16111-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16111-1-AP (COPG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human skeletal muscle using 16111-1-AP

    WB analysis of human skeletal muscle using 16111-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16111-1-AP (COPG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 16111-1-AP

    WB analysis of mouse brain using 16111-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16111-1-AP (COPG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 16111-1-AP

    WB analysis of mouse heart using 16111-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16111-1-AP (COPG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16111-1-AP

    WB analysis of HeLa using 16111-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16111-1-AP (COPG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 16111-1-AP

    WB analysis of mouse kidney using 16111-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16111-1-AP (COPG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 16111-1-AP

    IHC staining of mouse heart using 16111-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16111-1-AP (COPG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 16111-1-AP

    IHC staining of mouse heart using 16111-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16111-1-AP (COPG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 16111-1-AP

    IHC staining of mouse cerebellum using 16111-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16111-1-AP (COPG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 16111-1-AP

    IHC staining of mouse cerebellum using 16111-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16111-1-AP (COPG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 16111-1-AP

    IHC staining of human heart using 16111-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16111-1-AP (COPG2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 16111-1-AP

    IHC staining of human heart using 16111-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16111-1-AP (COPG2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human cerebellum using 16111-1-AP

    IHC staining of human cerebellum using 16111-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 16111-1-AP (COPG2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cerebellum using 16111-1-AP

    IHC staining of human cerebellum using 16111-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 16111-1-AP (COPG2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, human heart tissue, human skeletal muscle tissue, mouse brain tissue, mouse kidney tissue
Positive IHC detected inmouse heart tissue, human cerebellum tissue, human heart tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16111-1-AP targets COPG2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPG2 fusion protein Ag9034 相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit gamma 2
Calculated molecular weight 871 aa, 98 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC017443
Gene symbol COPG2
Gene ID (NCBI) 26958
RRIDAB_10897182
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COPG2 antibody 16111-1-APDownload protocol
IHC protocol for COPG2 antibody 16111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Identification and characterization of an endoplasmic reticulum localization motif.

Authors - Yong-Wei Nie
View Article