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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPE Monoclonal antibody (66994-1-Ig)

    WB analysis using 66994-1-Ig (same clone as 66994-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66994-1-Ig (COPE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66994-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using COPE Monoclonal antibody (66994-1-Ig)

    IF Staining of HEK-293 using 66994-1-Ig (same clone as 66994-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 66994-1-Ig (COPE antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66994-1-PBS in a different storage buffer formulation.

COPE Monoclonal antibody, PBS Only

COPE Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66994-1-PBS
Clone No.2B8E8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP, FLJ13241

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66994-1-PBS targets COPE in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28602

Product name: Recombinant human COPE protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-308 aa of BC017285

Sequence: MAPPAPGPASGGSGEVDELFDVKNAFYIGSYQQCINEAQRVKLSSPERDVERDVFLYRAYLAQRKFGVVLDEIKPSSAPELQAVRMFADYLAHESRRDSIVAELDREMSRSVDVTNTTFLLMAASIYLHDQNPDAALRALHQGDSLECTAMTVQILLKLDRLDLARKELKRMQDLDEDATLTQLATAWVSLATGGEKLQDAYYIFQEMADKCSPTLLLLNGQAACHMAQGRWEAAEGLLQEALDKDSGYPETLVNLIVLSQHLGKPPEVTNRYLSQLKDAHRSHPFIKEYQAKENDFDRLVLQYAPSA

相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit epsilon
Calculated molecular weight 34 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC017285
Gene Symbol COPE
Gene ID (NCBI) 11316
RRIDAB_2882311
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO14579
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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