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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COPB2 Polyclonal antibody (14733-1-AP)

    WB analysis using 14733-1-AP (same clone as 14733-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14733-1-AP (COPB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using COPB2 Polyclonal antibody (14733-1-AP)

    IHC staining of human stomach using 14733-1-AP (same clone as 14733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14733-1-AP (COPB2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using COPB2 Polyclonal antibody (14733-1-AP)

    IHC staining of human stomach cancer using 14733-1-AP (same clone as 14733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14733-1-AP (COPB2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14733-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using COPB2 Polyclonal antibody (14733-1-AP)

    IF Staining of PC-12 using 14733-1-AP (same clone as 14733-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using COPB2 antibody (14733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14733-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using COPB2 Polyclonal antibody (14733-1-AP)

    IF Staining of NIH/3T3 using 14733-1-AP (same clone as 14733-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using COPB2 antibody (14733-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14733-1-PBS in a different storage buffer formulation.

COPB2 Polyclonal antibody, PBS Only

COPB2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14733-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

p102, Coatomer subunit beta', Beta'-COP, Beta'-coat protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14733-1-PBS targets COPB2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6498

Product name: Recombinant human COPB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 557-906 aa of BC000326

Sequence: LDRTMYLLGYIPKDNRLYLGDKELNIISYSLLVSVLEYQTAVMRRDFSMADKVLPTIPKEQRTRVAHFLEKQGFKQQALTVSTDPEHRFELALQLGELKIAYQLAVEAESEQKWKQLAELAISKCQFGLAQECLHHAQDYGGLLLLATASGNANMVNKLAEGAERDGKNNVAFMSYFLQGKVDACLELLIRTGRLPEAAFLARTYLPSQVSRVVKLWRENLSKVNQKAAESLADPTEYENLFPGLKEAFVVEEWVKETHADLWPAKQYPLVTPNEERNVMEEGKDFQPSRSTAQQELDGKPASPTPVIVASHTANKEEKSLLELEVDLDNLELEDIDTTDINLDEDILDD

相同性解析による交差性が予測される生物種
Full Name coatomer protein complex, subunit beta 2 (beta prime)
Calculated molecular weight 102 kDa
Observed molecular weight102 kDa
GenBank accession numberBC000326
Gene Symbol COPB2
Gene ID (NCBI) 9276
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP35606
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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