Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 14754-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14754-1-AP (COMT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 14754-1-AP
IP result of anti-COMT (IP:14754-1-AP, 4ug; Detection:14754-1-AP 1:500) with K-562 cells lysate 1200ug.
IHC staining of human liver using 14754-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 14754-1-AP (COMT antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 14754-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14754-1-AP (COMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 14754-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14754-1-AP (COMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 14754-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14754-1-AP (COMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 14754-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14754-1-AP (COMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum using 14754-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (14754-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse cerebellum using 14754-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (14754-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 14754-1-AP
Immunofluorescent analysis of HepG2 cells, using COMT antibody 14754-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
FC experiment of PC-3 using 14754-1-AP
1x10^6 PC-3 cells were intracellularly stained with 0.8 ug COMT Polyclonal antibody (14754-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

COMT Polyclonal antibody

COMT Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 14754-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Synonyms

catechol O methyltransferase, Catechol O-methyltransferase, EC:2.1.1.6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (15)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, K-562 cells, human placenta, mouse liver, rat liver
Positive IP detected in	K-562 cells
Positive IHC detected in	human liver tissue, human placenta tissue, mouse lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse cerebellum tissue
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:800
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 13 publications below
IHC	See 2 publications below
IF	See 3 publications below

Product Information

14754-1-AP targets COMT in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6502 Product name: Recombinant human COMT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-182 aa of BC000419 Sequence: MNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAVRMARLLSPGARLITIEINPDCAAITQRMVDFAGMKDKVTLVVGASQDIIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDFLAHVRGSSCFECTHYQSFLEYREVVDGLEKAIYKGPGSEAGP 相同性解析による交差性が予測される生物種
Full Name	catechol-O-methyltransferase
Calculated molecular weight	30 kDa
Observed molecular weight	28-30 kDa
GenBank accession number	BC000419
Gene Symbol	COMT
Gene ID (NCBI)	1312
RRID	AB_2083706
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P21964
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

Protocols

Product Specific Protocols
IF protocol for COMT antibody 14754-1-AP	Download protocol
IHC protocol for COMT antibody 14754-1-AP	Download protocol
IP protocol for COMT antibody 14754-1-AP	Download protocol
WB protocol for COMT antibody 14754-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh) Meisoindigo Acts as a Molecular Glue to Target PKMYT1 for Degradation in Chronic Myeloid Leukemia Therapy Authors - Zhao-Xin Zhang View Article
mouse	WB	Aging (Albany NY) A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients. Authors - Xiao Wu View Article
mouse	WB	Aging (Albany NY) Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis. Authors - Dongdong Wang View Article
mouse	WB	Transl Psychiatry A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota. Authors - Yinrui Guo View Article
mouse	WB	FASEB J Entacapone alleviates acute kidney injury by inhibiting ferroptosis. Authors - Jiahong Yang View Article
human	WB,IHC	Endocrinology Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells. Authors - Saller S S View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14754-1-AP

IP experiment of K-562 using 14754-1-AP

IHC staining of human liver using 14754-1-AP

IHC staining of human placenta using 14754-1-AP

IHC staining of human placenta using 14754-1-AP

IHC staining of mouse lung using 14754-1-AP

IHC staining of mouse lung using 14754-1-AP

IF Staining of mouse cerebellum using 14754-1-AP

IF Staining of mouse cerebellum using 14754-1-AP

IF Staining of HepG2 using 14754-1-AP

FC experiment of PC-3 using 14754-1-AP

COMT Polyclonal antibody

COMT Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Meisoindigo Acts as a Molecular Glue to Target PKMYT1 for Degradation in Chronic Myeloid Leukemia Therapy

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.

Entacapone alleviates acute kidney injury by inhibiting ferroptosis.

Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells.