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Validation Data Gallery

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  • Western Blot (WB) analysis of pig cartilage tissue using COMP Monoclonal antibody (66793-1-Ig)

    WB analysis of pig cartilage using 66793-1-Ig (same clone as 66793-1-PBS)

    pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66793-1-Ig (COMP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COMP Monoclonal antibody (66793-1-Ig)

    IF Staining of HepG2 using 66793-1-Ig (same clone as 66793-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using COMP Monoclonal antibody (66793-1-Ig)

    IF Staining of U2OS using 66793-1-Ig (same clone as 66793-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

COMP Monoclonal antibody, PBS Only

COMP Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66793-1-PBS
Clone No.1D7A6

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

TSP5, 1D7A6, Cartilage oligomeric matrix protein, PSACH, Thrombospondin-5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66793-1-PBS targets COMP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4575

Product name: Recombinant human COMP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 407-757 aa of BC033676

Sequence: DNCPQKSNPDQADVDHDFVGDACDSDQDQDGDGHQDSRDNCPTVPNSAQEDSDHDGQGDACDDDDDNDGVPDSRDNCRLVPNPGQEDADRDGVGDVCQDDFDADKVVDKIDVCPENAEVTLTDFRAFQTVVLDPEGDAQIDPNWVVLNQGREIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQMEQTYWQANPFRAVAEPGIQLKAVKSSTGPGEQLRNALWHTGDTESQVRLLWKDPRNVGWKDKKSYRWFLQHRPQVGYIRVRFYEGPELVADSNVVLDTTMRGGRLGVFCFSQENIIWANLRYRCNDTIPEDYETHQLRQA

相同性解析による交差性が予測される生物種
Full Name cartilage oligomeric matrix protein
Calculated molecular weight 757 aa, 83 kDa
Observed molecular weight 100-130 kDa
GenBank accession numberBC033676
Gene Symbol COMP
Gene ID (NCBI) 1311
RRIDAB_2882137
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP49747
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others. This antibody got 100-130 kDa maybe due to glycosylation.