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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COMMD9 Recombinant antibody (84251-4-RR)

    WB analysis using 84251-4-RR (same clone as 84251-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84251-4-RR (COMMD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84251-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using COMMD9 Recombinant antibody (84251-4-RR)

    WB analysis of HEK-293 using 84251-4-RR (same clone as 84251-4-PBS)

    WB result of COMMD9 antibody (84251-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COMMD9 transfected HEK-293 cells. This data was developed using the same antibody clone with 84251-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84251-4-RR

    Affinity and Kinetic Characterization of 84251-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84251-4-RR against Human COMMD9 were performed. The affinity constant is 1.00 nM.

  • Featured Product
  • KD/KO Validated

COMMD9 Recombinant monoclonal antibody, PBS Only

COMMD9 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 84251-4-PBS
Clone No.241505B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

241505B4, C11orf55, COMM domain containing 9, COMM domain-containing protein 9, HSPC166

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84251-4-PBS targets COMMD9 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag35078

Product name: Recombinant human COMMD9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-198 aa of BC010892

Sequence: MAALTAEHFAALQSLLKASSKDVVRQLCQESFSSSALGLKKLLDVTCSSLSVTQEEAEELLQALHRLTRLVAFRDLSSAEAILALFPENFHQNLKNLLTKIILEHVSTWRTEAQANQISLPRLVDLDWRVDIKTSSDSISRMAVPTCLLQMKIQEDPSLCGDKPSISAVTVELSKETLDTMLDGLGRIRDQLSAVASK

相同性解析による交差性が予測される生物種
Full Name COMM domain containing 9
Observed molecular weight22 kDa
GenBank accession numberBC010892
Gene Symbol COMMD9
Gene ID (NCBI) 29099
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9P000
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

COMMD9, also known as HSPC166, is a member of the COMMD family of proteins. The COMMD protein family is an evolutionarily conserved gene family that plays a role in a number of critical biological processes, including inflammation, copper homeostasis, sodium balance, endosomal sorting and cancer. COMMD9 has been shown to participate in TFDP1/E2F1 activation and to play a critical role in non-small cell lung cancer (PMID: 27871936)

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