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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using COMMD8 Polyclonal antibody (25237-1-AP)

    WB analysis of HEK-293 using 25237-1-AP (same clone as 25237-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25237-1-AP (COMMD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using COMMD8 Polyclonal antibody (25237-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 25237-1-AP (same clone as 25237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25237-1-AP (COMMD8 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25237-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using COMMD8 Polyclonal antibody (25237-1-AP)

    IF Staining of HEK-293 using 25237-1-AP (same clone as 25237-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using COMMD8 antibody (25237-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 25237-1-PBS in a different storage buffer formulation.

COMMD8 Polyclonal antibody, PBS Only

COMMD8 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25237-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

COMM domain-containing protein 8, COMMD 8, MDS022

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25237-1-PBS targets COMMD8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18712

Product name: Recombinant human COMMD8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 9-182 aa of BC008371

Sequence: LWRLQKLPAELGPQLLHKIIDGICGRAYPVYQDYHTVWESEEWMHVLEDIAKFFKAIVGKNLPDEEIFQQLNQLNSLHQETIMKCVKSRKDEIKQALSREIVAISSAQLQDFDWQVKLALSSDKIAALRMPLLSLHLDVKENGEVKPYSIEMSREELQNLIQSLEAANKVVLQL

相同性解析による交差性が予測される生物種
Full Name COMM domain containing 8
Calculated molecular weight 183 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC008371
Gene Symbol COMMD8
Gene ID (NCBI) 54951
RRIDAB_2879980
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX08
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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