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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of mouse kidney using 10393-1-AP (same clone as 10393-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of human placenta using 10393-1-AP (same clone as 10393-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of human heart using 10393-1-AP (same clone as 10393-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of human stomach using 10393-1-AP (same clone as 10393-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse stomach tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of mouse stomach using 10393-1-AP (same clone as 10393-1-PBS)

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of mouse heart using 10393-1-AP (same clone as 10393-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of HEK-293 using 10393-1-AP (same clone as 10393-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of MCF-7 using 10393-1-AP (same clone as 10393-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    IP experiment of mouse heart using 10393-1-AP (same clone as 10393-1-PBS)

    IP result of anti-COMMD5 (IP:10393-1-AP, 4ug; Detection:10393-1-AP 1:500) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COMMD5 Polyclonal antibody (10393-1-AP)

    IF Staining of HeLa using 10393-1-AP (same clone as 10393-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COMMD5 antibody (10393-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10393-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

COMMD5 Polyclonal antibody, PBS Only

COMMD5 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 10393-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

COMM domain containing 5, COMM domain-containing protein 5, HCaRG, HT002, Hypertension-related calcium-regulated gene protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10393-1-PBS targets COMMD5 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0592

Product name: Recombinant human COMMD5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 4-159 aa of BC003055

Sequence: VGAATPYLHHPGDSHSGRVSFLGAQLPPEVAAMARLLGDLDRSTFRKLLKFVVSSLQGEDCREAVQRLGVSANLPEEQLGALLAGMHTLLQQALRLPPTSLKPDTFRDQLQELCIPQDLVGDLASVVFGSQRPLLDSVAQQQGAWLPHVADFRWRV

相同性解析による交差性が予測される生物種
Full Name COMM domain containing 5
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC003055
Gene Symbol COMMD5
Gene ID (NCBI) 28991
RRIDAB_2083555
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZQ3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.

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