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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COMMD4 Recombinant antibody (84664-4-RR)

    WB analysis using 84664-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84664-4-RR (COMMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using COMMD4 Recombinant monoclonal antibody (84664-4-RR)

    WB analysis of MCF-7 using 84664-4-RR

    WB result of COMMD4 antibody (84664-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COMMD4 transfected MCF-7 cells.

  • Immunohistochemistry (IHC) staining of rat testis tissue using COMMD4 Recombinant antibody (84664-4-RR)

    IHC staining of rat testis using 84664-4-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84664-4-RR (COMMD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using COMMD4 Recombinant antibody (84664-4-RR)

    IHC staining of rat testis using 84664-4-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84664-4-RR (COMMD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84664-4-RR

    Affinity and Kinetic Characterization of 84664-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84664-4-RR against Human COMMD4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

COMMD4 Recombinant monoclonal antibody

COMMD4 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84664-4-RR
Clone No.242175F4

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

242175F4, COMM domain containing 4, COMMD 4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells, HEK-293T cells, U2OS cells
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84664-4-RR targets COMMD4 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34904

Product name: Recombinant human COMMD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-199 aa of BC068998

Sequence: MRFRFCGDLDCPDWVLAEISTLAKMSSVKLRLLCSQVLKELLGQGIDYEKILKLTADAKFESGDVKATVAVLSFILSSAAKHSVDGESLSSELQQLGLPKEHAASLCRCYEEKQSPLQKHLRVCSLRMNRLAGVGWRVDYTLSSSLLQSVEEPMVHLRLEVAAAPGTPAQPVAMSLSADKFQVLLAELKQAQTLMSSLG

相同性解析による交差性が予測される生物種
Full Name COMM domain containing 4
Observed molecular weight25 kDa
GenBank accession numberBC068998
Gene Symbol COMMD4
Gene ID (NCBI) 54939
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9H0A8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COMMD4 is a protein-coding gene belonging to the COMMD family and is highly expressed in non-small cell lung cancer (NSCLC) and hepatocellular carcinoma (HCC) (PMID: 36249824). It has 3 isoforms and has been shown to control NF-κB activity and regulate the activity of cullin-RING E3 ubiquitin ligases (PMID: 32439936).

Protocols

Product Specific Protocols
IHC protocol for COMMD4 antibody 84664-4-RRDownload protocol
WB protocol for COMMD4 antibody 84664-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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