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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using COL6A3-Specific Recombinant antibody (83010-3-RR)

    FC experiment of HeLa using 83010-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Col6a3-Specific Recombinant Antibody (83010-3-RR, Clone:230540D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83010-3-RR

    Affinity and Kinetic Characterization of 83010-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83010-3-RR against Human COL6A3-Specific were performed. The affinity constant is 1.71 nM.

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Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83010-3-RR targets COL6A3 in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COL6A3 fusion protein Ag34752 相同性解析による交差性が予測される生物種
Full Name collagen, type VI, alpha 3
Calculated molecular weight 344 kDa
Observed molecular weight343 kDa
GenBank accession numberNM_004369
Gene Symbol COL6A3
Gene ID (NCBI) 1293
RRIDAB_3670755
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.

Protocols

Product Specific Protocols
FC protocol for COL6A3 antibody 83010-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols