Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
10807-1-AP targets COL4A6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COL4A6 fusion protein Ag1118 相同性解析による交差性が予測される生物種 |
| Full Name | collagen, type IV, alpha 6 |
| Calculated molecular weight | 1691 aa, 164 kDa |
| GenBank accession number | BC005305 |
| Gene Symbol | COL4A6 |
| Gene ID (NCBI) | 1288 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14031 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Collagen alpha-6(IV) chain, known as COL4A6, is a member of the collagen family, the major structural component of basement membranes. There are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network. COL4A6 forms a triple helical structure with two other alpha-5-chains, which is stabilized by the presence of glycine as every third residue (PMID: 27377778). COL4A6 is located on the X chromosome in a head-to-head manner with COL4A5 closely (PMID: 23714752). COL4A6 is transcribed from two alternative promoters in a tissue-specific fashion. This antibody detected the cleaved fragment of COL4A6 at ~50 kDa (PMID: 7657706)..
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COL4A6 antibody 10807-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene Hepatitis B virus X protein promotes the development of liver fibrosis and hepatoma through downregulation of miR-30e targeting P4HA2 mRNA. | ||
Polymers (Basel) The Development of a 3D PET Fibrous Scaffold Modified with an Umbilical Cord dECM for Liver Tissue Engineering | ||
Toxicol Appl Pharmacol High doses of baicalin induces kidney injury and fibrosis through regulating TGF-β/Smad signaling pathway. | ||
J Tissue Eng Regen Med Topographical and Chemical Inductive Cues Synergistically Enhance the Schwann Cell Differentiation of Aligned Dental Pulp Stem Cell Sheets | ||
J Immunol Res Partial Reconstruction of Uterus Cervix in Rat by Decellularized Human Uterine Cervical Scaffold Combined with Adipose-Derived Stem Cells (ADSCs) | ||
Oral Dis Artemisinin suppressed tumour growth and induced vascular normalisation in oral squamous cell carcinoma via inhibition of MIF |