Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 15191-1-AP (same clone as 15191-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 15191-1-AP (same clone as 15191-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.
WB analysis of human pancreas using 15191-1-AP (same clone as 15191-1-PBS)
human pancreas tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 15191-1-AP (same clone as 15191-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.
WB analysis of mouse pancreas using 15191-1-AP (same clone as 15191-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 15191-1-AP (same clone as 15191-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15191-1-AP (COL4A3BP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 15191-1-AP (same clone as 15191-1-PBS)
IP result of anti-COL4A3BP (IP:15191-1-AP, 4ug; Detection:15191-1-AP 1:300) with mouse brain tissue lysate 3680ug. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 15191-1-AP (same clone as 15191-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 15191-1-AP (same clone as 15191-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15191-1-AP (same clone as 15191-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15191-1-AP (same clone as 15191-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney slide using 15191-1-AP (COL4A3BP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 15191-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

COL4A3BP Polyclonal antibody, PBS Only

COL4A3BP Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 15191-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15191-1-PBS targets COL4A3BP in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7277 Product name: Recombinant human COL4A3BP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 372-598 aa of BC000102 Sequence: EEMVQNHMTYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLADNAIIIYQTHKRVWPASQRDVLYLSVIRKIPALTENDPETWIVCNFSVDHDSAPLNNRCVRAKINVAMICQTLVSPPEGNQEISRDNILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF 相同性解析による交差性が予測される生物種
Full Name	collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
Calculated molecular weight	71 kDa
Observed molecular weight	80-89 kDa
GenBank accession number	BC000102
Gene Symbol	COL4A3BP
Gene ID (NCBI)	10087
RRID	AB_2082664
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y5P4
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

COL4A3BP(Collagen type IV alpha-3-binding protein) is also named as CERT, STARD11. CERT extracts ceramide from the ER and carries it to the Golgi apparatus in a non-vesicular manner and that a particularly efficient cycle of CERT movement for trafficking of ceramide may proceed at membrane contact sites between the ER and the Golgi apparatus(PMID:17314061). This protein has 3 isoforms produced by alternative splicing.

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

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ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15191-1-AP (same clone as 15191-1-PBS)

WB analysis of rat brain using 15191-1-AP (same clone as 15191-1-PBS)

WB analysis of human pancreas using 15191-1-AP (same clone as 15191-1-PBS)

WB analysis of HEK-293 using 15191-1-AP (same clone as 15191-1-PBS)

WB analysis of mouse pancreas using 15191-1-AP (same clone as 15191-1-PBS)

WB analysis of mouse kidney using 15191-1-AP (same clone as 15191-1-PBS)

IP experiment of mouse brain using 15191-1-AP (same clone as 15191-1-PBS)

IHC staining of human pancreas using 15191-1-AP (same clone as 15191-1-PBS)