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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Collagen Type XVII Polyclonal antibody (31190-1-AP)

    WB analysis using 31190-1-AP (same clone as 31190-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31190-1-AP (COL17A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using Collagen Type XVII Polyclonal antibody (31190-1-AP)

    IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using Collagen Type XVII Polyclonal antibody (31190-1-AP)

    IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Collagen Type XVII Polyclonal antibody (31190-1-AP)

    IF Staining of A431 using 31190-1-AP (same clone as 31190-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using Collagen Type XVII Polyclonal antibody (31190-1-AP)

    IF Staining of HaCaT using 31190-1-AP (same clone as 31190-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

Collagen Type XVII Polyclonal antibody, PBS Only

Collagen Type XVII Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31190-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

COL17, COL17A1, LABD97, BP180, Collagen alpha 1(XVII) chain

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31190-1-PBS targets Collagen Type XVII in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34645

Product name: Recombinant human COL17A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC168368

Sequence: MDVTKKNKRDGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTHGSSGYINSTGSTRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTRHAYEGSSSGNSSPEYPRKEFASSSTRGRSQTRESEIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSSNTLPIPKKGTVETKIVTASS

相同性解析による交差性が予測される生物種
Full Name collagen, type XVII, alpha 1
Observed molecular weight180 kDa
GenBank accession numberBC168368
Gene Symbol COL17A1
Gene ID (NCBI) 1308
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UMD9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Collagen alpha-1(XVII) chain, also termed 180-kDa bullous pemphigoid antigen (BP180), is a type II transmembrane glycoprotein with a collagenous carboxyl-terminal extracellular domain and a noncollagenous amino-terminal cytoplasmic domain. High expression of COL17A1 has also been identified as a marker of human esophageal quiescent stem/progenitor cells (PMID: 35709398, 9545306). As a structural component of the hemidesmosome, COL17A1 is not only present as the full-length 180 kDa transmembrane form, but also as a cleaved 120 kDa soluble form that is further processed to a 97 kDa form (PMID: 113594550).

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