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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Collagen Type XIII Polyclonal antibody (19801-1-AP)

    WB analysis using 19801-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19801-1-AP (Collagen Type XIII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Collagen Type XIII Polyclonal antibody

Collagen Type XIII Polyclonal Antibody for WB, ELISA
Cat No. 19801-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

COL13, COL13A1, Collagen alpha 1(XIII) chain, Collagen alpha-1(XIII) chain, COLXIIIA1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHT-1080 cells, NIH/3T3 eclls

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19801-1-AP targets Collagen Type XIII in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name collagen, type XIII, alpha 1
Calculated molecular weight 70 kDa
Observed molecular weight62-70 kDa, 85-100 kDa
GenBank accession numberNM_001130103
Gene Symbol COL13A1
Gene ID (NCBI) 1305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5TAT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COL13A1 is involved in cell-matrix and cell-cell adhesion interactions that are required for normal development. COL13A1 may participate in the linkage between muscle fiber and basement membrane. COL13A1 may play a role in endochondral ossification of bone and branching morphogenesis of lung. COL13A1 can bind heparin. The antibody can recognize all the isoforms except isoform9 of COL13A1. This antibody is specific to COL13A1. It is predicted molecular weight is 62-70 kDa, and it is also reported as 85-95 kDa (PMID:11223332).

Protocols

Product Specific Protocols
WB protocol for Collagen Type XIII antibody 19801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols