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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COL12A1 Polyclonal antibody (30045-1-AP)

    WB analysis using 30045-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30045-1-AP (COL12A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using COL12A1 Polyclonal antibody (30045-1-AP)

    IF Staining of U2OS using 30045-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COL12A1 antibody (30045-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Collagen Type XII Polyclonal antibody

Collagen Type XII Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30045-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

COL12, COL12A1, COL12A1L, Collagen alpha 1(XII) chain, Collagen alpha-1(XII) chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, SKOV-3 cells, C2C12 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30045-1-AP targets Collagen Type XII in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type XII fusion protein Ag32602 相同性解析による交差性が予測される生物種
Full Name collagen, type XII, alpha 1
Calculated molecular weight333kd
Observed molecular weight310-333 kDa
GenBank accession numberNM_004370
Gene Symbol COL12A1
Gene ID (NCBI) 1303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99715
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COL12A1 (Collagen alpha-1(XII) chain), also known as COL12A1L. It is predicted to be located in the ECM. Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix. The molecular weight of COL12A1 is 333 kDa.

Protocols

Product Specific Protocols
WB protocol for Collagen Type XII antibody 30045-1-APDownload protocol
IF protocol for Collagen Type XII antibody 30045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols