Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, ROS1728 cells, HCT 116 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
| IHC | See 8 publications below |
| IF | See 5 publications below |
Product Information
26984-1-AP targets Collagen Type X in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25741 Product name: Recombinant human COL10A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 518-585 aa of BC130621 Sequence: QAVMPEGFIKAGQRPSLSGTPLVSANQGVTGMPVSAFTVILSKAYPAIGTPIPFDKILYNRQQHYDPR 相同性解析による交差性が予測される生物種 |
| Full Name | collagen, type X, alpha 1 |
| Calculated molecular weight | 680 aa, 66 kDa |
| Observed molecular weight | 60-66 kDa |
| GenBank accession number | BC130621 |
| Gene Symbol | COL10A1 |
| Gene ID (NCBI) | 1300 |
| RRID | AB_3085918 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q03692 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Collagen type X alpha 1 (COL10A1), a secreted, short-chain collagen, belongs to the collagen family, which is a major interstitial matrix component specifically expressed by hypertrophic chondrocytes. COL10A1 expression is elevated in many solid tumor types, such as colon cancer, esophagus cancer, and breast cancer, and displays vital roles in many critical cellular processes (PMID: 30154451, 25321476).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Collagen Type X antibody 26984-1-AP | Download protocol |
| WB protocol for Collagen Type X antibody 26984-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Biodegradable Self-Enhancing Piezoelectric Scaffold for Synchronized Tendon-to-Bone Regeneration. | ||
Bioact Mater Bioinspired scaffold recapitulating chondrogenic ontogeny and microenvironment for functional cartilage regeneration. | ||
Bioact Mater Immune-modulated adhesive hydrogel for enhancing osteochondral graft adhesion and cartilage repair | ||
Adv Sci (Weinh) PIEZO1-GPX4 Axis Mediates Mechanical Stress-Induced Vertebral Growth Plate Dysplasia via Ferroptosis Activation | ||
Biomaterials Dual-loaded BMSCs-Metformin microgels promote cartilage repair through combined anti-inflammatory, antioxidant, and regenerative effects. | ||
Int J Biol Sci Mettl1-mediated m7G modification of Fgfr2 regulates osteogenic and chondrogenic differentiation of mesenchymal stem cells |