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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Coilin Recombinant monoclonal antibody (87171-1-RR)

    WB analysis using 87171-1-RR (same clone as 87171-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87171-1-RR (COIL antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87171-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Coilin Recombinant monoclonal antibody (87171-1-RR)

    WB analysis using 87171-1-RR (same clone as 87171-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87171-1-RR (COIL antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87171-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Coilin Recombinant monoclonal antibody (87171-1-RR)

    WB analysis of HEK-293 using 87171-1-RR (same clone as 87171-1-PBS)

    WB result of Coilin antibody (87171-1-RR; 1:300000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Coilin transfected HEK-293 cells. This data was developed using the same antibody clone with 87171-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02891-1

    Sandwich ELISA sample test of MP02891-1

    The mean Coilin concentration was determined to be 19.9 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load and 9.6 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02891-1

    Sandwich ELISA standard curve of MP02891-1

    Sandwich ELISA standard curve of MP02891-1, Human Coilin Recombinant Matched Antibody Pair - PBS only. 87171-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1416. 87171-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Coilin Recombinant monoclonal antibody (87171-1-RR)

    IF Staining of HepG2 using 87171-1-RR (same clone as 87171-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COIL antibody (87171-1-RR, Clone: 252327B11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87171-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02891-1

    Cytometric bead array sample test of MP02891-1

    The mean COIL/Coilin concentration was determined to be 63.3 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 29.8 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 88.3 ng/mL in K-562 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02891-1

    Cytometric bead array standard curve of MP02891-1

    Cytometric bead array standard curve of MP02891-1, COIL/Coilin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87171-2-PBS. Detection antibody: 87171-1-PBS. Standard: Ag1416. Range: 0.312-40 ng/mL.

  • Affinity of 87171-1-RR

    Affinity of 87171-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87171-1-RR against Human Coilin were performed. The affinity constant is below 1 pM.

Coilin Recombinant monoclonal antibody, PBS Only (Detector)

Coilin Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 87171-1-PBS
Clone No.252327B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

COIL, CLN80, p80 coilin, p80-coilin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87171-1-PBS targets Coilin as part of a matched antibody pair:

MP02891-1: 87171-2-PBS capture and 87171-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1416

Product name: Recombinant human COIL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 212-576 aa of BC010385

Sequence: ANQRCSSPKGSARNSLVKAKRKGSVSVCSKESPSSSSESESCDESISDGPSKVTLEARNSSEKLPTELSKEEPSTKNTTADKLAIKLGFSLTPSKGKTSGTTSSSSDSSAESDDQCLMSSSTPECAAGFLKTVGLFAGRGRPGPGLSSQTAGAAGWRRSGSNGGGQAPGASPSVSLPASLGRGWGREENLFSWKGAKGRGMRGRGRGRGHPVSCVVNRSTDNQRQQQLNDVVKNSSTIIQNPVETPKKDYSLLPLLAAAPQVGEKIAFKLLELTSSYSPDVSDYKEGRILSHNPETQQVDIEILSSLPALREPGKFDLVYHNENGAEVVEYAVTQESKITVFWKELIDPRLIIESPSNTSSTEPA

相同性解析による交差性が予測される生物種
Full Name coilin
Calculated molecular weight 576 aa, 63 kDa
Observed molecular weight75 kDa
GenBank accession numberBC010385
Gene Symbol COIL
Gene ID (NCBI) 8161
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP38432
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs.

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