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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COG8 Polyclonal antibody (12661-1-AP)

    WB analysis using 12661-1-AP (same clone as 12661-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12661-1-AP (COG8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using COG8 Polyclonal antibody (12661-1-AP)

    IHC staining of mouse testis using 12661-1-AP (same clone as 12661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12661-1-AP (COG8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using COG8 Polyclonal antibody (12661-1-AP)

    IHC staining of human ovary cancer using 12661-1-AP (same clone as 12661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12661-1-AP (COG8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12661-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using COG8 Polyclonal antibody (12661-1-AP)

    IF Staining of A431 using 12661-1-AP (same clone as 12661-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using COG8 antibody (12661-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12661-1-PBS in a different storage buffer formulation.

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COG8 Polyclonal antibody, PBS Only

COG8 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12661-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

COG complex subunit 8, DOR1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12661-1-PBS targets COG8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COG8 fusion protein Ag3341 相同性解析による交差性が予測される生物種
Full Name component of oligomeric golgi complex 8
Calculated molecular weight 612 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC017492
Gene Symbol COG8
Gene ID (NCBI) 84342
RRIDAB_2244993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MW5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The conserved oligomeric Golgi (COG) complex is an eight subunit (COG1-8) peripheral Golgi membrane hetero-oligomeric protein complex. The COG complex is organized into lobes A (COG2-4) and B (COG5-7) with COG1 and COG8 bridging these lobes and is thought to play a critical role in vesicle tethering processes (PMID: 17331980). Cog8 localizes exclusively in the Golgi apparatus and is involved in the retrograde Golgi transport of resident proteins responsible for sugar chain (glycan) biosynthesis (PMID: 17220172).