Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, Y79 cells, HeLa cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13552-1-AP targets COG7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4335 Product name: Recombinant human COG7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 419-770 aa of BC037563 Sequence: CGLLSALKSLFAKYVSDFTSTLQSIRKKCKLDHIPPNSLFQEDWTAFQNSIRIIATCGELLRHCGDFEQQLANRILSTAGKYLSDSCSPRSLAGFQESILTDKKNSAKNPWQEYNYLQKDNPAEYASLMEILYTLKEKGSSNHNLLAAPRAALTRLNQQAHQLAFDSVFLRIKQQLLLISKMDSWNTAGIGETLTDELPAFSLTPLEYISNIGQYIMSLPLNLEPFVTQEDSALELALHAGKLPFPPEQGDELPELDNMADNWLGSIARATMQTYCDAILQIPELSPHSAKQLATDIDYLINVMDALGLQPSRTLQHIVTLLKTRPEDYRQVSKGLPRRLATTVATMRSVNY 相同性解析による交差性が予測される生物種 |
| Full Name | component of oligomeric golgi complex 7 |
| Calculated molecular weight | 770 aa, 86 kDa |
| Observed molecular weight | 86 kDa |
| GenBank accession number | BC037563 |
| Gene Symbol | COG7 |
| Gene ID (NCBI) | 91949 |
| RRID | AB_2229608 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P83436 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COG7 (Component of Oligomeric Golgi Complex 7) is a critical protein-coding gene located on chromosome 16p12.2. By maintaining the structure and environment of the Golgi, COG7 is essential for the proper attachment of sugar chains (glycosylation) to proteins and lipids.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for COG7 antibody 13552-1-AP | Download protocol |
| WB protocol for COG7 antibody 13552-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |