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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COG7 Polyclonal antibody (13552-1-AP)

    WB analysis using 13552-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13552-1-AP (COG7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using COG7 Polyclonal antibody (13552-1-AP)

    WB analysis of Y79 using 13552-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13552-1-AP (COG7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using COG7 Polyclonal antibody (13552-1-AP)

    IHC staining of human cervical cancer using 13552-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13552-1-AP (COG7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using COG7 Polyclonal antibody (13552-1-AP)

    IHC staining of human cervical cancer using 13552-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13552-1-AP (COG7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COG7 Polyclonal antibody

COG7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13552-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CDG2E, COG complex subunit 7, COG7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Y79 cells, HeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13552-1-AP targets COG7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4335

Product name: Recombinant human COG7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 419-770 aa of BC037563

Sequence: CGLLSALKSLFAKYVSDFTSTLQSIRKKCKLDHIPPNSLFQEDWTAFQNSIRIIATCGELLRHCGDFEQQLANRILSTAGKYLSDSCSPRSLAGFQESILTDKKNSAKNPWQEYNYLQKDNPAEYASLMEILYTLKEKGSSNHNLLAAPRAALTRLNQQAHQLAFDSVFLRIKQQLLLISKMDSWNTAGIGETLTDELPAFSLTPLEYISNIGQYIMSLPLNLEPFVTQEDSALELALHAGKLPFPPEQGDELPELDNMADNWLGSIARATMQTYCDAILQIPELSPHSAKQLATDIDYLINVMDALGLQPSRTLQHIVTLLKTRPEDYRQVSKGLPRRLATTVATMRSVNY

相同性解析による交差性が予測される生物種
Full Name component of oligomeric golgi complex 7
Calculated molecular weight 770 aa, 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC037563
Gene Symbol COG7
Gene ID (NCBI) 91949
RRIDAB_2229608
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP83436
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COG7 antibody 13552-1-APDownload protocol
IHC protocol for COG7 antibody 13552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols