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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using COG6 Polyclonal antibody (14277-1-AP)

    WB analysis of human brain using 14277-1-AP (same clone as 14277-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COG6 Polyclonal antibody (14277-1-AP)

    WB analysis using 14277-1-AP (same clone as 14277-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using COG6 Polyclonal antibody (14277-1-AP)

    WB analysis of NIH/3T3 using 14277-1-AP (same clone as 14277-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using COG6 Polyclonal antibody (14277-1-AP)

    IHC staining of human stomach cancer using 14277-1-AP (same clone as 14277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14277-1-AP (COG6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using COG6 Polyclonal antibody (14277-1-AP)

    IHC staining of human stomach cancer using 14277-1-AP (same clone as 14277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14277-1-AP (COG6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using COG6 Polyclonal antibody (14277-1-AP)

    IF Staining of MCF-7 using 14277-1-AP (same clone as 14277-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COG6 Polyclonal antibody (14277-1-AP)

    IF Staining of HeLa using 14277-1-AP (same clone as 14277-1-PBS)

    Immunofluorescent analysis of Hela cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14277-1-PBS in a different storage buffer formulation.

COG6 Polyclonal antibody, PBS Only

COG6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14277-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14277-1-PBS targets COG6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5624

Product name: Recombinant human COG6 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 308-657 aa of BC051723

Sequence: VGDMLAWLHQATASEKEHLEALLKHVTTQGVEENIQEVVGHITEGVCRPLKVRIEQVIVAEPGAVLLYKISNLLKFYHHTISGIVGNSATALLTTIEEMHLLSKKIFFNSLSLHASKLMDKVELPPPDLGPSSALNQTLMLLREVLASHDSSVVPLDARQADFVQVLSCVLDPLLQMCTVSASNLGTADMATFMVNSLYMMKTTLALFEFTDRRLEMLQFQIEAHLDTLINEQASYVLTRVGLSYIYNTVQQHKPEQGSLANMPNLDSVTLKAAMVQFDRYLSAPDNLLIPQLNFLLSATVKEQIVKQSTELVCRAYGEVYAAVMNPINEYKDPENILHRSPQQVQTLLS

相同性解析による交差性が予測される生物種
Full Name component of oligomeric golgi complex 6
Calculated molecular weight 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC051723
Gene Symbol COG6
Gene ID (NCBI) 57511
RRIDAB_10694426
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2V7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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