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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COG4 Polyclonal antibody (31539-1-AP)

    WB analysis using 31539-1-AP (same clone as 31539-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31539-1-AP (COG4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31539-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COG4 Polyclonal antibody (31539-1-AP)

    WB analysis using 31539-1-AP (same clone as 31539-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31539-1-AP (COG4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using COG4 Polyclonal antibody (31539-1-AP)

    IHC staining of mouse testis using 31539-1-AP (same clone as 31539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31539-1-AP (COG4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31539-1-PBS in a different storage buffer formulation.

COG4 Polyclonal antibody, PBS Only

COG4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31539-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

COD1, COG complex subunit 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31539-1-PBS targets COG4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35295

Product name: Recombinant human COG4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 506-785 aa of NM_001365426.1

Sequence: FQDIQRGVTSAVNIMHSSLQQGKFDTKGIESTDEAKMSFLVTLNNVEVCSENISTLKKTLESDCTKLFSQGIGGEQAQAKFDSCLSDLAAVSNKFRDLLQEGLTELNSTAIKPQVQPWINSFFSVSHNIEEEEFNDYEANDPWVQQFILNLEQQMAEFKASLSPVIYDSLTGLMTSLVAVELEKVVLKSTFNRLGGLQFDKELRSLIAYLTTVTTWTIRDKFARLSQMATILNLERVTEILDYWGPNSGPLTWRLTPAEVRQVLALRIDFRSEDIKRLRL

相同性解析による交差性が予測される生物種
Full Name component of oligomeric golgi complex 4
Calculated molecular weight89kDa，785aa
Observed molecular weight89 kDa
GenBank accession numberNM_001365426.1
Gene Symbol COG4
Gene ID (NCBI) 25839
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9H9E3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

COG complex subunit 4 is present in high molecular weight complexes that govern membrane movement between the Golgi and ER. Cog4 dependent trafficking and secretion of ECM components are required for protrusion and extension of the epithelial projections of the inner ear (PMID: 30287385). Loss-of-function COG4-mutant cells have impaired glycosylation and reduced COG4 protein levels (PMID: 30290151).