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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COG1 Polyclonal antibody (10767-1-AP)

    WB analysis using 10767-1-AP (same clone as 10767-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10767-1-AP (COG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10767-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using COG1 Polyclonal antibody (10767-1-AP)

    WB analysis using 10767-1-AP (same clone as 10767-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10767-1-AP (COG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10767-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using COG1 Polyclonal antibody (10767-1-AP)

    IHC staining of human ovary tumor using 10767-1-AP (same clone as 10767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10767-1-AP (COG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10767-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using COG1 Polyclonal antibody (10767-1-AP)

    IHC staining of human ovary tumor using 10767-1-AP (same clone as 10767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10767-1-AP (COG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10767-1-PBS in a different storage buffer formulation.

COG1 Polyclonal antibody, PBS Only

COG1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10767-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10767-1-PBS targets COG1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1203

Product name: Recombinant human COG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 561-980 aa of BC021985

Sequence: ADAGTVQEMLRTQSVACIKHIVDCIRAELQSIEEGVQGQQDALNSAKLHSVLFMARLCQSLGELCPHLKQCILGKSESSEKPAREFRALRKQGKVKTQEIIPTQAKWQEVKEVLLQQSVMGYQVWSSAVVKVLIHGFTQSLLLDDAGSVLATATSWDELEIQEEAESGSSVTSKIRLPAQPSWYVQSFLFSLCQEINRVGGHALPKVTLQEMLKSCMVQVVAAYEKLSEEKQIKKEGAFPVTQNRALQLLYDLRYLNIVLTAKGDEVKSGRSKPDSRIEKVTDHLEALIDPFDLDVFTPHLNSNLHRLVQRTSVLFGLVTGTENQLAPRSSTFNSQEPHNILPLASSQIRFGLLPLSMTSTRKAKSTRNIETKAQVVPPARSTAGDPTVPGSLFRQLVSEEDNTSAPSLFKLGWLSSMTK

相同性解析による交差性が予測される生物種
Full Name component of oligomeric golgi complex 1
Calculated molecular weight 109 kDa
Observed molecular weight 109 kDa
GenBank accession numberBC021985
Gene Symbol COG1
Gene ID (NCBI) 9382
RRIDAB_2083677
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WTW3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells. It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa and western blot analysis detected COG1 at 100-109 kDa (PMID: 16537452).

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