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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COBLL1 Polyclonal antibody (31191-1-AP)

    WB analysis using 31191-1-AP (same clone as 31191-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 31191-1-AP (COBLL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using COBLL1 Polyclonal antibody (31191-1-AP)

    IHC staining of human placenta using 31191-1-AP (same clone as 31191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using COBLL1 Polyclonal antibody (31191-1-AP)

    IHC staining of human placenta using 31191-1-AP (same clone as 31191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31191-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using COBLL1 Polyclonal antibody (31191-1-AP)

    IHC staining of mouse kidney using 31191-1-AP (same clone as 31191-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31191-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using COBLL1 Polyclonal antibody (31191-1-AP)

    IF Staining of HaCaT using 31191-1-AP (same clone as 31191-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using COBLL1 antibody (31191-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31191-1-PBS in a different storage buffer formulation.

COBLL1 Polyclonal antibody, PBS Only

COBLL1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31191-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

COBL like 1, COBLR1, Cordon bleu protein like 1, KIAA0977

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31191-1-PBS targets COBLL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34853

Product name: Recombinant human COBLL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 990-1128 aa of BC071588

Sequence: LAVVKRSQSFSKERTESPSASALVQPPANTEEGKTHSVNKFVDIPQLGVSDKENNSAHNEQNSQIPTPTDGPSFTVMRQSSLTFQSSDPEQMRQSLLTAIRSGEAAAKLKRVTIPSNTISVNGRSRLSHSMSPDAQDGH

相同性解析による交差性が予測される生物種
Full Name COBL-like 1
Observed molecular weight160 kDa
GenBank accession numberBC071588
Gene Symbol COBLL1
Gene ID (NCBI) 22837
RRIDAB_3669891
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ53SF7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cordon-blue protein-like 1 (COBLL1), a negative regulator of apoptosis, is associated with lower insulin resistance. COBLL1 is involved in the cancer cell morphogenesis to a neuron-like cell shape observed in the CRPC model cells, promoting cell growth and migration (PMID: 29686105, 29686105). COBLL1 is highly expressed in most human tissues including lung, liver, kidney, pancreas, ovary, spinal cord and brain.

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