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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COA4 Polyclonal antibody (30721-1-AP)

    WB analysis using 30721-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30721-1-AP (COA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using COA4 Polyclonal antibody (30721-1-AP)

    WB analysis using 30721-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 30721-1-AP (COA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using COA4 Polyclonal antibody (30721-1-AP)

    IHC staining of human ovary cancer using 30721-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30721-1-AP (COA4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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COA4 Polyclonal antibody

COA4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30721-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CHCHD8, E2IG2, E2 induced gene 2 protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30721-1-AP targets COA4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COA4 fusion protein Ag33966 相同性解析による交差性が予測される生物種
Full Name Cytochrome c oxidase assembly factor 4 homolog, mitochondrial
Calculated molecular weight10 kDa
Observed molecular weight12 kDa
GenBank accession numberNM_016565.2
Gene Symbol COA4
Gene ID (NCBI) 51287
RRIDAB_3669754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYJ1-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The coiled-coil-helix-coiled-coil-helix domain containing 8 (CHCHD8) is a putative COX assembly factor known as cytochrome c oxidase assembly factor 4 homolog (CoA4). COA4 is a newly identified CcO assembly factor which is a twin CX(9)C motif mitochondrial protein localized in the intermembrane space linked with the inner membrane of mitochondria. Its transport into intermembrane space depends on the MIA40 trans-site receptor machinery. (PMID: 37345060)

Protocols

Product Specific Protocols
WB protocol for COA4 antibody 30721-1-APDownload protocol
IHC protocol for COA4 antibody 30721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols