Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25197-1-AP targets COA1 in IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18313 Product name: Recombinant human C7orf44 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-146 aa of BC056884 Sequence: MMWQKYAGSRRSMPLGARILFHGVFYAGGFAIVYYLIQKFHSRALYYKLAVEQLQSHPEAQEALGPPLNIHYLKLIDRENFVDIVDAKLKIPVSGSKSEGLLYVHSSRGGPFQRWHLDEVFLELKDGQQIPVFKLSGENGDEVKKE 相同性解析による交差性が予測される生物種 |
| Full Name | Cytochrome c oxidase assembly factor 1 homolog |
| Calculated molecular weight | 146 aa, 17 kDa |
| GenBank accession number | BC056884 |
| Gene Symbol | COA1 |
| Gene ID (NCBI) | 55744 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZY4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COA1, also named as C7orf44 and MITRAC15, is a mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein with MW 15 kDa. It's a component of some MITRAC complex, a cytochrome c oxidase (COX) assembly intermediate complex that regulates COX assembly. COA1 is required for assembly of mitochondrial respiratory chain complex I and complex IV.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COA1 antibody 25197-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |