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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNTLN Polyclonal antibody (31698-1-AP)

    WB analysis using 31698-1-AP (same clone as 31698-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 31698-1-AP (CNTLN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31698-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CNTLN Polyclonal antibody (31698-1-AP)

    IHC staining of human liver using 31698-1-AP (same clone as 31698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31698-1-AP (CNTLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31698-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CNTLN Polyclonal antibody (31698-1-AP)

    IF Staining of HeLa using 31698-1-AP (same clone as 31698-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNTLN antibody (31698-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31698-1-PBS in a different storage buffer formulation.

CNTLN Polyclonal antibody, PBS Only

CNTLN Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31698-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C9orf101, C9orf39, Centlein, Centrosomal protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31698-1-PBS targets CNTLN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag36354

Product name: Recombinant human CNTLN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 150-380 aa of

Sequence: REKQKSEAKDRKVLEILQVKDAKIQEFEQRESVLKQEINDLVKRKIAVDEENAFLRKEFSDLEKKFKDKSQEIKDTKECVQNKEEQNRLVIKNLEEENKKLSTRCTDLLNDLEKLRKQEAHLRKEKYSTDAKIKTFEDNLIEARKEVEVSQSKYNALSLQLSNKQTELIQKDMDITLVRKELQELQNLYKQNSTHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESISY

相同性解析による交差性が予測される生物種
Full Name centlein, centrosomal protein
Calculated molecular weight162kDa
Observed molecular weight162 kDa
Gene Symbol CNTLN
Gene ID (NCBI) 54875
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NXG0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CNTLN is also named C9orf101 and C9orf39. CNTLN is required for centrosome cohesion and recruitment of CEP68 to centrosomes. CNTLN is localized at the centrosome. The calculated molecular weight of CNTLN is 162 kDa.

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