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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNTD2 Polyclonal antibody (31588-1-AP)

    WB analysis using 31588-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31588-1-AP (CNTD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CNTD2 Polyclonal antibody (31588-1-AP)

    WB analysis of HepG2 using 31588-1-AP

    WB result of CNTD2 antibody (31588-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNTD2 transfected HepG2 cells.

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  • Featured Product
  • KD/KO Validated

CNTD2 Polyclonal antibody

CNTD2 Polyclonal Antibody for WB, ELISA
Cat No. 31588-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Homo sapiens (Human), Cyclin-P , Cyclin P

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31588-1-AP targets CNTD2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNTD2 fusion protein Ag36380 相同性解析による交差性が予測される生物種
Full Name cyclin N-terminal domain containing 2
Calculated molecular weight33kDa
Observed molecular weight25 kDa
GenBank accession numberNM_024877
Gene Symbol CNTD2
Gene ID (NCBI) 79935
RRIDAB_3670047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9H8S5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNTD2, also known as Cyclin N-terminal domain containing 2 or CCNP, is a protein that has been implicated in cancer cell proliferation and migration, particularly in colon and lung cancers . It is an atypical cyclin that contains a cyclin box domain, which is a characteristic feature of cyclin-dependent kinase (CDK) binding partners. CNTD2 is considered an atypical cyclin due to its structural specificities, which were revealed through the human genome sequence project (PMID: 30087414). The apparent molecular mass measured by SDS-PAGE is 25 kDa.

Protocols

Product Specific Protocols
WB protocol for CNTD2 antibody 31588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols