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CNPase Monoclonal antibody
CNPase Monoclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Pig, mouse and More (1)
Applications
WB, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1B9G9
Cat no : 66729-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | pig cerebellum tissue, U-251 cells, human brain tissue, HepG2 cells, rat brain tissue, rat cerebellum tissue |
| Positive IF detected in | mouse brain tissue |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
66729-1-Ig targets CNPase in WB, IF, FC, ELISA applications and shows reactivity with Human, Rat, Pig, mouse samples.
| Tested Reactivity | Human, Rat, Pig, mouse |
| Cited Reactivity | rat, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CNPase fusion protein Ag4227 相同性解析による交差性が予測される生物種 |
| Full Name | 2',3'-cyclic nucleotide 3' phosphodiesterase |
| Calculated molecular weight | 421 aa, 48 kDa |
| Observed molecular weight | 45-48 kDa |
| GenBank accession number | BC028040 |
| Gene symbol | CNP |
| Gene ID (NCBI) | 1267 |
| RRID | AB_2882079 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CNPase antibody 66729-1-Ig | Download protocol |
| IF protocol for CNPase antibody 66729-1-Ig | Download protocol |
| FC protocol for CNPase antibody 66729-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
CNS Neurosci Ther EAAT3 impedes oligodendrocyte remyelination in chronic cerebral hypoperfusion-induced white matter injury | ||
J Ethnopharmacol Shenzhiling oral liquid protects the myelin sheath against Alzheimer's disease through the PI3K/Akt-mTOR pathway. |
