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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNP Recombinant monoclonal antibody (86967-4-RR)

    WB analysis using 86967-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86967-4-RR (CNP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CNP Recombinant monoclonal antibody (86967-4-RR)

    WB analysis using 86967-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86967-4-RR (CNP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CNP Recombinant monoclonal antibody (86967-4-RR)

    IHC staining of mouse brain using 86967-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86967-4-RR (CNP antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CNPase Recombinant monoclonal antibody (86967-4-RR)

    IF Staining of mouse brain using 86967-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CNPase antibody (86967-4-RR, Clone: 252105D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CNPase Recombinant monoclonal antibody (86967-4-RR)

    IF Staining of mouse brain using 86967-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CNPase antibody (86967-4-RR, Clone: 252105D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CNPase Recombinant monoclonal antibody

CNPase Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86967-4-RR
Clone No.252105D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

CNP, 2',3' cyclic nucleotide 3' phosphodiesterase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, EC:3.1.4.37

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue, A375 cells, A431 cells, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86967-4-RR targets CNPase in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4227

Product name: Recombinant human CNP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-421 aa of BC028040

Sequence: MNRGFSRKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTVATLLECKTLFILRGLPGSGKSTLARVIVDKYRDGTKMVSADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIRILVLDDTNHERERLEQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII

相同性解析による交差性が予測される生物種
Full Name 2',3'-cyclic nucleotide 3' phosphodiesterase
Calculated molecular weight 421 aa, 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC028040
Gene Symbol CNPase
Gene ID (NCBI) 1267
RRIDAB_3745239
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CNPase antibody 86967-4-RRDownload protocol
IHC protocol for CNPase antibody 86967-4-RRDownload protocol
WB protocol for CNPase antibody 86967-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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