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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of Jurkat using 10752-1-AP (same clone as 10752-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of HeLa using 10752-1-AP (same clone as 10752-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of mouse testis using 10752-1-AP (same clone as 10752-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of mouse testis using 10752-1-AP (same clone as 10752-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IP experiment of mouse testis using 10752-1-AP (same clone as 10752-1-PBS)

    IP result of anti-CNOT8 (IP:10752-1-AP, 4ug; Detection:10752-1-AP 1:600) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human prostate cancer using 10752-1-AP (same clone as 10752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10752-1-AP (CNOT8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human prostate cancer using 10752-1-AP (same clone as 10752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human prostate cancer using 10752-1-AP (same clone as 10752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human kidney using 10752-1-AP (same clone as 10752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human kidney using 10752-1-AP (same clone as 10752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CNOT8 Polyclonal antibody (10752-1-AP)

    IF Staining of HeLa using 10752-1-AP (same clone as 10752-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNOT8 antibody (10752-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10752-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CNOT8 Polyclonal antibody, PBS Only

CNOT8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10752-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CALIF, CAF2, CAF1-like protein, Caf1b, CAF1 like protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10752-1-PBS targets CNOT8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1201

Product name: Recombinant human CNOT8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-292 aa of BC017366

Sequence: MPAALVENSQVICEVWASNLEEEMRKIREIVLSYSYIAMDTEFPGVVVRPIGEFRSSIDYQYQLLRCNVDLLKIIQLGLTFTNEKGEYPSGINTWQFNFKFNLTEDMYSQDSIDLLANSGLQFQKHEEEGIDTLHFAELLMTSGVVLCDNVKWLSFHSGYDFGYMVKLLTDSRLPEEEHEFFHILNLFFPSIYDVKYLMKSCKNLKGGLQEVADQLDLQRIGRQHQAGSDSLLTGMAFFRMKELFFEDSIDDAKYCGRLYGLGTGVAQKQNEDVDSAQEKMSILAIINNMQQ

相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 8
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC017366
Gene Symbol CNOT8
Gene ID (NCBI) 9337
RRIDAB_2082470
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UFF9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.

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