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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CNOT4 Polyclonal antibody

CNOT4 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12564-1-AP

Synonyms

CCR4 associated factor 4, CLONE243, CNOT4, NOT4, NOT4H

Validation Data Gallery

Applications:
  • WB analysis of mouse skeletal muscle using 12564-1-AP

    WB analysis of mouse skeletal muscle using 12564-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12564-1-AP (CNOT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 12564-1-AP

    WB analysis of MCF-7 using 12564-1-AP

    WB result of CNOT4 antibody (12564-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT4 transfected MCF-7 cells.

  • WB analysis using 12564-1-AP

    WB analysis using 12564-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12564-1-AP (CNOT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 12564-1-AP

    WB analysis of mouse testis using 12564-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12564-1-AP (CNOT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12564-1-AP

    IHC staining of human lung cancer using 12564-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12564-1-AP (CNOT4 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CNOT4 antibody included
  • IF Staining of HepG2 using 12564-1-AP

    IF Staining of HepG2 using 12564-1-AP

    Immunofluorescent analysis of HepG2 cells, using CNOT4 antibody 12564-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • FC experiment of HepG2 using 12564-1-AP

    FC experiment of HepG2 using 12564-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug CNOT4 antibody (12564-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT4 Ready-To-Use IHC Kit. CNOT4 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

12564-1-AP targets CNOT4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT4 fusion protein Ag3163 相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 4
Calculated molecular weight 575 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC035590
Gene symbol CNOT4
Gene ID (NCBI) 4850
RRIDAB_2082457
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CNOT4 antibody 12564-1-APDownload protocol
IHC protocol for CNOT4 antibody 12564-1-APDownload protocol
IF protocol for CNOT4 antibody 12564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Sci

Coupled protein quality control during nonsense-mediated mRNA decay

Authors - Alison J Inglis
View Article
humanWB,IF

Cells

Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability

Authors - Nafiseh Chalabi Hagkarim
  • KD Validated
View Article