Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83824-4-RR targets CNNM4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag5121 Product name: Recombinant human CNNM4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 420-775 aa of BC063295 Sequence: DILYVKDLAFVDPDDCTPLKTITRFYNHPVHFVFHDTKLDAMLEEFKKGKSHLAIVQKVNNEGEGDPFYEVLGLVTLEDVIEEIIKSEILDESDMYTDNRSRKRVSEKNKRDFSAFKDADNELKVKISPQLLLAAHRFLATEVSQFSPSLISEKILLRLLKYPDVIQELKFDEHNKYYARHYLYTRNKPADYFILILQGKVEVEAGKENMKFETGAFSYYGTMALTSVPSDRSPAHPTPLSRSASLSYPDRTDVSTAATLAGSSNQFGSSVLGQYISDFSVRALVDLQYIKITRQQYQNGLLASRMENSPQFPIDGCTTHMENLAEKSELPVVDETTTLLNERNSLLHKASHENAI 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin M4 |
| Calculated molecular weight | 87 kDa |
| Observed molecular weight | 90-100 kDa |
| GenBank accession number | BC063295 |
| Gene Symbol | CNNM4 |
| Gene ID (NCBI) | 26504 |
| RRID | AB_3671408 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6P4Q7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CNNM4 antibody 83824-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |