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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CNNM4 Polyclonal antibody (14066-1-AP)

    WB analysis of HEK-293 using 14066-1-AP (same clone as 14066-1-PBS)

    WB result of CNNM4 antibody (14066-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNNM4 transfected HEK-293 cells. This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CNNM4 Polyclonal antibody (14066-1-AP)

    WB analysis using 14066-1-AP (same clone as 14066-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14066-1-AP (CNNM4 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CNNM4 Polyclonal antibody (14066-1-AP)

    IP experiment of mouse testis using 14066-1-AP (same clone as 14066-1-PBS)

    IP result of anti-CNNM4 (IP:14066-1-AP, 4ug; Detection:14066-1-AP 1:1000) with mouse testis tissue lysate 4400ug. This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CNNM4 Polyclonal antibody (14066-1-AP)

    IHC staining of human colon using 14066-1-AP (same clone as 14066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14066-1-AP (CNNM4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CNNM4 Polyclonal antibody (14066-1-AP)

    IF Staining of MCF-7 using 14066-1-AP (same clone as 14066-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CNNM4 antibody (14066-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CNNM4 Polyclonal antibody, PBS Only

CNNM4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14066-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ACDP4, Ancient conserved domain-containing protein 4, cyclin M4, Cyclin-M4, KIAA1592

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14066-1-PBS targets CNNM4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5121

Product name: Recombinant human CNNM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 420-775 aa of BC063295

Sequence: DILYVKDLAFVDPDDCTPLKTITRFYNHPVHFVFHDTKLDAMLEEFKKGKSHLAIVQKVNNEGEGDPFYEVLGLVTLEDVIEEIIKSEILDESDMYTDNRSRKRVSEKNKRDFSAFKDADNELKVKISPQLLLAAHRFLATEVSQFSPSLISEKILLRLLKYPDVIQELKFDEHNKYYARHYLYTRNKPADYFILILQGKVEVEAGKENMKFETGAFSYYGTMALTSVPSDRSPAHPTPLSRSASLSYPDRTDVSTAATLAGSSNQFGSSVLGQYISDFSVRALVDLQYIKITRQQYQNGLLASRMENSPQFPIDGCTTHMENLAEKSELPVVDETTTLLNERNSLLHKASHENAI

相同性解析による交差性が予測される生物種
Full Name cyclin M4
Calculated molecular weight 87 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC063295
Gene Symbol CNNM4
Gene ID (NCBI) 26504
RRIDAB_2878009
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P4Q7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).

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