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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNGA4 Polyclonal antibody (22201-1-AP)

    WB analysis using 22201-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22201-1-AP (CNGA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CNGA4 Polyclonal antibody

CNGA4 Polyclonal Antibody for WB, ELISA
Cat No. 22201-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

CNCA2, CNG5, CNGA4, CNGB2, OCNC2, OCNCb, OCNCBETA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22201-1-AP targets CNGA4 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNGA4 fusion protein Ag17481 相同性解析による交差性が予測される生物種
Full Name cyclic nucleotide gated channel alpha 4
Calculated molecular weight 575 aa, 66 kDa
Observed molecular weight47-50 kDa, 65-70 kDa
GenBank accession numberBC106936
Gene Symbol CNGA4
Gene ID (NCBI) 1262
RRIDAB_3669391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IV77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNGA4 (cyclic nucleotide gated channel subunit alpha 4), also known as CNG4. It is expected to be located in cell membrane. Cyclic nucleotide-gated (CNG) channels are crucial for visual and olfactory transductions (PMID: 12432397). Native CNG channels are heterotetramers composed of homologous A and B subunits. Olfactory CNG channels are composed of three types of subunits, CNGA2, CNGA4, and CNGB1b (PMID: 15134638). CNGA4 is a modulatory subunit which contributes to the high cAMP sensitivity of the native olfactory channel. By accelerating the calcium-mediated negative feedback in olfactory signaling it allows rapid adaptation of native olfactory neurons to odorants (PMID: 11739959; 11739960). The molecular weight of CNGA4 is 65 kDa, and it can recognize the 48 kDa isoform.

Protocols

Product Specific Protocols
WB protocol for CNGA4 antibody 22201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols