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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CMPK2 Polyclonal antibody

CMPK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25877-1-AP

Synonyms

CMPK2, TYKi, UMP CMPK2

Validation Data Gallery

Applications:
  • WB analysis using 25877-1-AP

    WB analysis using 25877-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25877-1-AP (CMPK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 25877-1-AP

    IHC staining of human lung cancer using 25877-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25877-1-AP (CMPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

25877-1-AP targets CMPK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CMPK2 fusion protein Ag22885 相同性解析による交差性が予測される生物種
Full Name cytidine monophosphate (UMP-CMP) kinase 2, mitochondrial
Calculated molecular weight 449 aa, 49 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC141802
Gene symbol CMPK2
Gene ID (NCBI) 129607
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CMPK2 belongs to the thymidylate kinase family. CMPK2 may participate in dUTP and dCTP synthesis in mitochondria. It is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy of CMPK2 is highest for dUMP followed by dCMP; CMP and UMP are poor substrates. CMPK2 may be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs.

Protocols

Product Specific Protocols
WB protocol for CMPK2 antibody 25877-1-APDownload protocol
IHC protocol for CMPK2 antibody 25877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Biol Sci

The FTO-CMPK2 Pathway in Fibroblast-like Synoviocytes Modulates Rheumatoid Arthritis Synovial Inflammation and Cartilage Homeostasis via mtDNA Regulation

Authors - Li Jin
View Article