Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67519-1-Ig targets CMPK1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30084 Product name: Recombinant human CMPK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-228 aa of BC014961 Sequence: MLSRCRSRLLHVLGLSFLLQTRRPILLCSPRLMKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG 相同性解析による交差性が予測される生物種 |
| Full Name | cytidine monophosphate (UMP-CMP) kinase 1, cytosolic |
| Calculated molecular weight | 228 aa, 26 kDa |
| Observed molecular weight | 22-26 kDa |
| GenBank accession number | BC014961 |
| Gene Symbol | CMPK1 |
| Gene ID (NCBI) | 51727 |
| RRID | AB_2882739 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P30085 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CMPK1 antibody 67519-1-Ig | Download protocol |
| WB protocol for CMPK1 antibody 67519-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
