Validation Data Gallery

Filter: AllWB

WB analysis using 17409-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 17409-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17409-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17409-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 17409-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

CMBL Polyclonal antibody

CMBL Polyclonal Antibody for WB, ELISA

Cat No. 17409-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CMBL

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

HeLa cells, mouse liver tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

17409-1-AP targets CMBL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11442 Product name: Recombinant human CMBL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC001573 Sequence: MANEAYPCPCDIGHRLEYGGLGREVQVEHIKAYVTKSPVDAGKAVIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVGQEPWDPSGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHAQKIGIVGFCWGGTAVHHLMMKYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEYQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLNKYM 相同性解析による交差性が予測される生物種
Full Name	carboxymethylenebutenolidase homolog (Pseudomonas)
Calculated molecular weight	245 aa, 28 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC001573
Gene Symbol	CMBL
Gene ID (NCBI)	134147
RRID	AB_10596776
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96DG6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CMBL antibody 17409-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Cell Rep p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP Authors - Yingdan Huang View Article

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Target

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Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17409-1-AP

WB analysis of mouse kidney using 17409-1-AP

WB analysis of mouse liver using 17409-1-AP

WB analysis of HeLa using 17409-1-AP

WB analysis of mouse skeletal muscle using 17409-1-AP