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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLYBL Recombinant antibody (85399-4-RR)

    WB analysis using 85399-4-RR (same clone as 85399-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85399-4-RR (CLYBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85399-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CLYBL Recombinant antibody (85399-4-RR)

    WB analysis of HSC-T6 using 85399-4-RR (same clone as 85399-4-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 85399-4-RR (CLYBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85399-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85399-4-RR

    Affinity and Kinetic Characterization of 85399-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85399-4-RR against Human CLYBL were performed. The affinity constant is below 1 pM.

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CLYBL Recombinant antibody, PBS Only

CLYBL Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85399-4-PBS
Clone No.242785F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

(3S)-malyl-CoA thioesterase, Beta-methylmalate synthase, Citramalyl-CoA lyase, mitochondrial, citrate lyase beta like, Citrate lyase beta-like

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85399-4-PBS targets CLYBL in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLYBL fusion protein Ag11283 相同性解析による交差性が予測される生物種
Full Name citrate lyase beta like
Calculated molecular weight 340 aa, 37 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC034360
Gene Symbol CLYBL
Gene ID (NCBI) 171425
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N0X4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CLYBL, also named CLB, is a Mitochondrial citramalyl-CoA lyase indirectly involved in vitamin B12 metabolism (PubMed:29056341). It is a human mitochondrial enzyme of unknown function that is found in multiple eukaryotic taxa and conserved to bacteria. It acts as a beta-methylmalate synthase in vitro, by mediating the conversion of glyoxylate and propionyl-CoA to beta-methylmalate. There are two isoforms with MW 31-33 kDa and 35-37 kDa.