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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLPTM1 Polyclonal antibody (15337-1-AP)

    WB analysis using 15337-1-AP (same clone as 15337-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15337-1-AP (CLPTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15337-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using CLPTM1 Polyclonal antibody (15337-1-AP)

    IP experiment of A549 using 15337-1-AP (same clone as 15337-1-PBS)

    IP result of anti-CLPTM1 (IP:15337-1-AP, 4ug; Detection:15337-1-AP 1:300) with A549 cells lysate 1050 ug. This data was developed using the same antibody clone with 15337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CLPTM1 Polyclonal antibody (15337-1-AP)

    IHC staining of mouse brain using 15337-1-AP (same clone as 15337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15337-1-AP (CLPTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CLPTM1 Polyclonal antibody (15337-1-AP)

    IHC staining of mouse brain using 15337-1-AP (same clone as 15337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15337-1-AP (CLPTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15337-1-PBS in a different storage buffer formulation.

CLPTM1 Polyclonal antibody, PBS Only

CLPTM1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15337-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Putative lipid scramblase CLPTM1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15337-1-PBS targets CLPTM1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7538

Product name: Recombinant human CLPTM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC004865

Sequence: MAAAQEADGARSAVVAAGGGSSGQVTSNGSIGRDPPAETQPQNPPAQPAPNAWQVIKGVLFRIFIIWAISSWFRRGPAPQDQAGPGGAPRVASRNLFPKDTLMNLHVYISEHEHFTDFNATSALFWEQHDLVYGDWTSGENSDGCYEHFAELDIPQSVQQNGSIYIHVYFTKSGFHPDPRQKALYRRLATVHMSRMINKYKRRRFQKTKNLLTGETEADPEMIKRAEDYGPVEVISHWHPNITINIVDDHTPWVKGSVPPPLDQYVKFDAVSGDYYPIIYFNDYWNLQKDYYPINESLASLPLRVSFCPLSLWRWQLYAAQSTKSPWNFLGDELYEQSDEEQDSVKVALLE

相同性解析による交差性が予測される生物種
Full Name cleft lip and palate associated transmembrane protein 1
Calculated molecular weight 62 kDa
Observed molecular weight 65-75 kDa
GenBank accession numberBC004865
Gene Symbol CLPTM1
Gene ID (NCBI) 1209
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO96005
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cleft lip and palate transmembrane protein 1 (CLPTM1), a multipass transmembrane protein, was identified due to the association of a chromosomal translocation with a cleft lip and palate (PMID: 9828125). CLPTM1 regulates epileptic seizures by modulating GABAAR-mediated inhibitory synaptic transmission (PMID: 36519412). Highly expressed CLPTM1L may contribute to a poor prognosis and increase invasion, proliferation and migration of oral squamous cell carcinoma (PMID: 34586883).

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