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Validation Data Gallery

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  • Western Blot (WB) analysis of rat pancreas tissue using CLPS Recombinant antibody (84224-2-RR)

    WB analysis of rat pancreas using 84224-2-RR

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 84224-2-RR (CLPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using CLPS Recombinant antibody (84224-2-RR)

    IHC staining of mouse pancreas using 84224-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using CLPS Recombinant antibody (84224-2-RR)

    IHC staining of mouse pancreas using 84224-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84224-2-RR

    Affinity and Kinetic Characterization of 84224-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84224-2-RR against Human CLPS were performed. The affinity constant is below 1 pM.

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CLPS Recombinant antibody

CLPS Recombinant Antibody for WB, IHC, ELISA
Cat No. 84224-2-RR
Clone No.241565H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Colipase, 241565H3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84224-2-RR targets CLPS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLPS fusion protein Ag2321 相同性解析による交差性が予測される生物種
Full Name colipase, pancreatic
Calculated molecular weight 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC017897
Gene Symbol CLPS
Gene ID (NCBI) 1208
RRIDAB_3671777
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04118
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts.

Protocols

Product Specific Protocols
WB protocol for CLPS antibody 84224-2-RRDownload protocol
IHC protocol for CLPS antibody 84224-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols